1. Write an equation showing the reaction of TRIS in the
acid form with sodium hydroxide (do not write out the chemical formula for
2. What is the pH of a solution that is 0.1 M in TRIS in the
acid form and 0.05 M in TRIS in the basic form?
3. Explain why you do not need to know the chemical formula
of a buffer compound to use it.
4. If you have a HEPES buffer at pH 4.75, will it be a
usable buffer? Why or why not?