1. Write an equation showing the reaction of TRIS in the acid form with sodium hydroxide (do not…

1. Write an equation showing the reaction of TRIS in the
acid form with sodium hydroxide (do not write out the chemical formula for
TRIS).

2. What is the pH of a solution that is 0.1 M in TRIS in the
acid form and 0.05 M in TRIS in the basic form?

 3. Explain why you do not need to know the chemical formula
of a buffer compound to use it.

4. If you have a HEPES buffer at pH 4.75, will it be a
usable buffer? Why or why not?